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UPPAAL (Larsen et al. 1997) tool has a feature of urgent locations, which are essentially locations with invariant clock value (le)0.
The ratio invariant value for each region is shown in color (shades between blue to red).
We used the default values of one cold and three heated Markov chains with invariant sites and gamma distribution (model GTR+I+G, allowing sites to vary independently) according to Modeltest v. 3.6 (Posada & Crandall 1998).
Values were normalized to the mean expression of two different internal reference genes (Mitofusin 1 and Thioredoxin 1), with invariant abundance in our experimental conditions.
The Kimura two-parameter model with invariant sites was chosen for both the tree reconstruction and distance calculations based on the likelihood ratio test of log likelihood values provided using the nucleotide model test also implemented in Mega.
Havran, W. L., Carbone, A. & Allison, J. P. Murine T cells with invariant γδ antigen receptors: origin, repertoire, and specificity.
Examining both variant and invariant loci, values varied from πTorishima = 0.00060 to πTern = 0.00065 (Table S2).
The (G, h -invariant boundary values of th -invariant can boundaryposed with the observaluesduced profecthens.
Our results are consistent with those of Monsalve et al. (2006), suggesting an approximately invariant velocity value for the upper crust.
Second, we simulated 100 data sets using the NH3 model with a Gamma + invariant rate distribution, with parameter values estimated from the real data set and the same number of sites.
Modulus values for materials exhibiting minimal time-dependent responses were found to agree well with known values and to be invariant of experimental conditions.
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