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For all panels, additional Sanger sequencing was performed for any region with insufficient depth of coverage for reliable heterozygous variant detection.
A saturation analysis was first performed on each preprocessed and aligned sample (BAM file) to ensure sufficient coverage depth (and therefore reproducibility): any sample with insufficient depth (based on saturation correlation <0.90) was excluded from further analyses.
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This study was partly motivated by the limited representation of immune-related transcripts in our original skin assembly, together with the fact that many of these had insufficient depth of sequence coverage to allow SNPs to be identified with high confidence.
Another factor that predisposes to recurrent LPD is trochlear dysplasia with insufficient trochlear depth, which is present in up to 85% of patients with recurrent patellar dislocation [ 10].
For example, the KIAA1549 BRAF fusion is often expressed at relatively low levels and may be missed with insufficient sequencing depth (authors' own observations).> In general, PAs are considered to have an excellent prognosis with overall 10-year survival reported to be over 90%% [ 9].
Third, nucleotide positions with read depth less than 5 were treated as ambiguous sites since there is insufficient depth to make a high confidence call [ 8].
Creative compromise Sometimes, you'll find that there is insufficient depth of field to focus clearly on the entirety of your frame.
Samples sequenced to a depth of less than 7 M such reads were excluded, having insufficient depth for complete recovery of P. copri ORFs (see 'Materials and methods').
For the genes identified as having lower expression in microarrays but not found in MPSS, it is possible that this may have been caused by running the MPSS experiments with insufficient sampling depths resulting in a less representative sampling of tags.
This reflects insufficient depth-coverage.
The ascertainment bias (the systematic distortion in measuring the true frequency of SNPs due to sampling) introduced in the marker development phase may have selected markers with insufficient variation outside V. vinifera or phylogenetic depth, and differences may be distorted due to domestication.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com