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In addition to 10 µg luciferase construct containing Rb1 or PTEN 3'UTR was transfected in each well, cells were also transfected with increasing folds of VerUTR plasmids and corresponding amounts of control vector to a total of 2 µg plasmid per well.
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Similar conclusions were drawn when we plotted the frequency of genes with increasing fold change.
Furthermore, we plotted the frequency of genes with increasing fold change values.
However, these data do demonstrate that the increase in relative error with increasing fold changes may be a common feature of multiple microarray platforms.
Complexes formed on radiolabelled DBA/2J or 129B6 probes were competed with increasing fold molar excess of either unlabelled DBA/2J DNA or unlabelled 129B6 DNA.
The healthy follicles show increasing gene variation with increasing fold difference for the subset of genes which are differentially regulated between healthy and atretic follicles, which is not seen in the atretic follicle group.
Finally, examination of dimm mRNA levels by qPCR revealed that increasing Pe dose correlated with increased fold change in transcript.
In addition, these studies focused on genes with increased fold change at 30 min and this single time point may also yield an incomplete list of ciliogenesis genes.
A more recent study found that overexpression of HSP70 enhanced Kv1.5 levels, consistent with increased folding and trafficking, whereas DNAJB1 had no effect (Hirota et al., 2008); as with CFTR and hERG, other DNAJs might be more active.
In a recent analysis of chaperones, knockdown and overexpression showed that HSP90β, HSC70 and DNAJA1 enhance KCNQ4 levels, consistent with increased folding; however, HSP90α diminished KCNQ4 levels (Table 2).
This increase is largely correlated with an increased folding rate constant, and with a smaller but significant decrease in the unfolding rate constant.
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