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The F2 animals genotyped with the 9K low density panel were imputed to 60K with imputation accuracy of approximately 0.99, as previously described (Gualdron Duarte et al. 2013).
The genotypes of control subjects were imputed using the same 1000 Genomes Project reference panel, with imputation performed using MaCH software (8).
Missing headache days data were imputed using a modified last observation carried forward (mLOCF) methodology, with imputation applied chronologically.
We excluded poorly imputed polymorphisms (with r < 0.3), and deleted individual genotypes with imputation quality scores <0.9.
Some sporadic missing genotypes were imputed using the BEAGLE v4.0 procedure [ 63], only SNPs with imputation quality score R > 0.5 were retained.
Some sporadic missing genotypes were imputed using the BEAGLE v4.0 procedure [ 66], then only SNPs with imputation quality score R > 0.5 underwent the next analyses step.
Obviously, causality is not suspended, nor it is replaced with imputation.
[15], focuses on estimating β, α, and the nonparametric function (g(cdot)) with imputation method when the covariate X is measured with additive error and the response variable Y is sometimes missing in the model (1.1).
We performed a genome-wide association scan with imputation.
The authors would like to thank Yongtao Guan for help with imputation software and data formatting.
For each significant SNP a functional proxy was searched with imputation, without improving the results.
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