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Frozen tumors were manually homogenized with homogenization hammer and tissue powders were resuspended in 500 μl of PBS.
This homogenate was diluted with homogenization buffer (HB; 0.25 M sucrose, 10 mM Hepes, 1 mM EDTA, pH 7.3) containing 1.5 mM glycyl-l-phenylalanine 2-naphthylamide and 1% dimethyl sulfoxide (DMSO).
Fecal and cecal content samples (~ 50 mg) were subjected to three consecutive freeze thaws and directly extracted by precooled phosphate buffer with homogenization using the Precellys Tissue Homogenizer.
Primary tumors were homogenized in a glass tissue grinder with homogenization buffer before extraction with the RNeasy MiniKit.
"In a global age when people are bombarded with homogenization, the last reservoir for genuine products and European culture is the mountain communities".
The dense white ZG band was collected, pooled, and washed with homogenization buffer by recentrifugation.
Similar(18)
The homogenate was then diluted with the homogenization medium to ultimately yield 10% (v/v).
Total RNA was extracted from samples with TRIzol® (Invitrogen, USA), in combination with tissue homogenization by means of ceramic beads (PRECELLYS, Berthin Pharma).
The mean particle diameter decreased with increasing homogenization pressure and number of passes, with a linear log log relationship between mean particle diameter and homogenization pressure.
RNA was extracted using Tri-Reagent, with tissue homogenization performed using a Mini-BeadBeater 8 (BioSpec) with silica beads (0.175 mg) at full speed for 30s.
At once thoroughly cosmopolitan yet at heart consummately Japanese, Ando's universally grounded architecture suggests that globalization need not equate with cultural homogenization.
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CEO of Professional Science Editing for Scientists @ prosciediting.com