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SLiMChance takes advantage of this feature of SLiMBuild to combine accurate predictions with high stringency.
Among the 159 novel miRNAs, 42 were identified with high stringency in our data set.
Blots were washed twice with low stringency buffer at room temperature and twice with high stringency buffer at 45°C.
Prehybridization temperature and hybridization temperature were 50°C, and the incubation temperature with high stringency buffer was 65°C.
Since the plasma proteomic data were filtered with high stringency, there were potentially a lot of false negative proteins.
Using the functional annotation clustering with high stringency, these genes fell within 4 major functional groups including development, glycoproteins, signal transduction, and transcriptional factors (Figure S7).
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The hits with high stringencies (alignment score ≥ 1000) were considered as duplications.
Efficiency was favored for jobs with many input sequences, suggesting high coverage that will be sufficient to create an accurate assembly even with high stringencies for alignment.
As a consequence, association studies can be performed with higher stringency.
For the gene families with paralogs, additional BLASTCLUST procedures with higher stringency were implemented to discover the true orthologs.
Third, associations between publications and gene communities can be calculated with higher stringency using the edge-swapping null-model in conjunction with bi-binomial P-values as compared to binomial or hypergeometric statistics.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com