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Both PBS and the mixed solutions were purged with high pure nitrogen (99.999%) for 30 min prior to measuring.
Total DNA was extracted from peripheral blood with High Pure PCR Template Preparation Kit (Roche Diagnostics GmbH, Mannheim, Germany).
The sample for the fluorescence measurement was dissolved in the dry toluene, filtered, transferred to a long quartz cell, and then capped and bubbled with high pure argon (without O2and moisture) for at least 15 min before measurement.
To fabricate a microstructural surface, cleaned Si wafer was placed on a translation stage in a vacuum chamber filled with high pure SF6 gas at a pressure of 5 × 104 Pa.
RNA extraction was carried out with High Pure RNA isolation kit (Roche) according to manufacturer's instructions.
Plasmid DNA was purified with High Pure Plasmid Isolation Kit (Roche).
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The FMWCNT blend membranes appeared to be more hydrophilic, with higher pure water flux than did the pure PPSU and MWCNT/PPSU blend membranes.
The teflon coated tube was washed with high-pure water and wiped clean before each test.
Throughout most direct antiviral agent (DAA) approval studies, HCV RNA cutoffs and endpoints were established with the COBAS TaqMan assay for use with the High Pure System (HPS/CTM).
Due to changes in the central laboratory, two assays were used to quantify HCV RNA, the COBAS TaqMan HCV Test, V2.0 for use with the High Pure System (Roche Molecular Systems, West Sussex, UK) with a lower limit of quantification (LLOQ) of 25 IU/mL and the COBAS AmpliPrep/COBAS TaqMan HCV Test v. 2.0 with an LLOQ of 15 IU/mL.
The PCR products were purified with the High Pure PCR Product Purification Kit (Roche Diagnostics, Tokyo, Japan) and then sequenced directly with the ABI Prism BigDye Terminator v1.1 Cycle Sequencing Kit (Life Technologies).
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