A stopped-flow spectrofluorometer was used to study the kinetics renaturation of nuclease from the acidified form on neutralization, the refolding is fast and the data can be described as a sequence of two first-order processes with half times of about 55 and 350 milliseconds, respectively.
While the weak interactions are completed within milliseconds, the formation of strong covalent bonds often proceeds with half-times on the order of minutes or hours.
PIH analogs undergo significant amino acid-catalyzed hydrolysis in cell culture medium and in serum, achieving equilibrium with their corresponding aldehydes and hydrazides with half-times of 1 8 h.
Their uptake curves for mannitol were interpreted as showing a single component with half-time of about 15-20 min. Their time course for retention of mannitol had a half-life of a few hours, perhaps similar to the half-life of about 4 h found for sucrose by Groothuis et al. as discussed in section 4.1.
The elimination kinetics of wollastonite fibers from the lung were relatively fast, with half-times of 15 to 21 days.
The red and green chromophores of E2-Red/Green mature quickly with half-times of 1.2 h and 0.4 h, respectively.
Depending on the animal species studied, particle type, and particle load, inorganic materials are ingested by macrophages on alveolar surfaces with half-times of 0.6 to 7 hr.
Moreover, a characterization of the GFP-albumin dynamics in HeLa cells based on fluorescence recovery after photobleaching (FRAP) (Patterson et al., 2008), showed that this construct enters and exits the Golgi with half-times of about 3 4 min and diffuses 'horizontally' along the Golgi ribbon in seconds, as expected from its soluble nature.
The fluorescent signal in the centre of a particle with a radius of ≈4 µm recovered with a half time of ≈40 s.
Cholesterol in this process turns over with a half time of approximately 24 h [15].
Once formed, patches disassembled with a half time of approximately 15 hr upon Snf7 washout.
