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The biotransformations were carried out with growing cells (standard screening conditions) and with non-growing cells.
Traditionally, biosynthesis of Monascus pigments is regarded as cell-growth dependence and production of Monascus pigments is usually carried out by microbial fermentation with growing cells (Chen et al. 2015).
Further analysis by direct incubation of the soluble extract with growing cells indicated that components from the extract preferentially bound to HeLa cells, but not to MC3T3 cells, which might help explain the observed preference of HeLa cells on the nanofibrillar scaffold.
Supernatants from wells with growing cells were screened for antibodies reactive to Aβ. Isotype determination was performed using the IsoQuick™ strips for Mouse Monoclonal Isotyping (Sigma-Aldrich, Germany).
Until now, yeast eQTL studies have been performed with growing cells.
Recent studies have shown that cells undergoing OIS have higher OXPHOS activities compared with growing cells (Quijano et al., 2012; Dorr et al., 2013; Kaplon et al., 2013).
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The authors would like to thank Lili Chen and Judy Cheung for FACS analysis, David Stathas, Usha Prasad, Amber Rivera, and Lise Hyung for support with growing cell lines and making FFPE blocks and slides, and Thomas Sherwood for tumor pathology analysis.
The experiments have been carried out with exponentially growing cells and with cells in early and late plateau phase.
However, comprehensive understanding of ECM and its dynamic relation with the growing cells is vital for creating functional tissue models ex vivo.
However, unlike the current experiments with logarithmically growing cells, spontaneous mutation rates were determined in stationary phase cells over a period of 14 days [12], [15].
Variation of results with actively growing cells was significant and, if anything, it was observed that Ecs deficiency slightly inhibited autolysis of cells during the exponential phase.
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