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For HIV-uninfected cells that are transfected with gag DNA, Gag expression is greatly enhanced by codon optimization [5]-[11].
"They settled them with gag orders," she added.
Fisa requests come with gag orders, meaning Google, Facebook and other tech companies cannot say whether they have received them.
They are served not to the targets of investigation but to communications providers or banks, and come with gag orders preventing the recipient from disclosing anything about them.
TRKN-mutated CCL5 had lower propensity for colocalization with GAG in the Golgi compared to the WT chemokine.
FISH experiments showed GAPDH mRNA, an abundant endogenous mRNA, colocalized with Gag at the PM and at the periphery of the Gag-enriched vacuoles (Fig. 5A).
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The non-availability of this ligand to gp150+ MuHV-4 fitted with GAG-dependent cell binding preceding gp150 displacement.
Positive control samples were generated using co-transfection of pNL4-3Δgag-protease with gag-protease amplified from pNL4-3, pHXB2 (subtype B), and pMJ4 (subtype C).
CMV pp65-specific CD8+ T cells had a greater proportion of the more mature LM (CD27−CD28−) cells compared with Gag-specific CD8+ T cells.
Recent work has shown the reduced replication capacity of chimeric viruses with gag-protease from LTNP-EC in comparison with chronically infected individuals [28].
Gag protein production was quantified by measuring the concentration of Gag p24 in cell supernatant by ELISA and by staining transfected cells with Gag-p24 specific fluorescent antibody.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com