Sentence examples for with further extraction from inspiring English sources

The sample was transferred to centrifuge bottles (250 ml) and centrifuged at 6000g for 10 min. The supernatant was collected, and the soil pellet re-extracted with further extraction buffer (50 ml), incubation at 65°C for 10 minutes and centrifuged as above.

The sample was incubated at 65°C for 1 hr, transferred to centrifuge bottles (250 ml) and centrifuged at 6000g for 10 min. The supernatant was collected, and the soil pellet re-extracted with further extraction buffer (100 ml), incubation at 65°C for 10 minutes and centrifugation as above.

To support the defined process we present the metamodels that can be integrated with existing DSL (domain-specific language) frameworks to extend them with information supporting further extraction of fault propagation behavior.

Further extraction with water will extract high polarity of compounds such as phenolic acids, sugars and glycosides [ 59– 61].

Heisey [ 15, 20] found that the water extract of A. altissima leaves inhibites the growth of Lepidium satium (garden cress), and the activity of the water extract was not affected by further extraction with methylene chloride, but was greatly affected by extraction with methanol.

After further extraction with hot phenol/chloroform and digestion with proteinase K (PK, 200 μg ml−1 (Sambrook et al, 1989)) total nucleic acids were precipitated and stored in 1 ml of ethanol containing 0.3 MM sodium acetate (pH 5.2) at −70°C.

Recovery of hydrolysates and further extraction with KOH were done as in (b).

The resulting residue was air dried and used for further extraction with sterile H2O at 80°C.

RNA extraction was performed according to TRIzol instructions, followed by DNAseI (RNase-free; Invitrogen Life Technologies) treatment to remove contaminating genomic DNA, and further extraction with phenol-chloroform-isoamylalcohol (25 : 25 : 1).

The NP40-insoluble fraction contains all components of the tubulin-based cytoskeleton but further extraction with salt solubilises the subpellicular microtubules, therefore the NP40/1M NaCl-insoluble fraction contains only the highly insoluble flagella.

In order to confirm this, further extraction experiments with pulverized cell matter could be carried out.