Those factors significantly associated with survival were further analyzed with the Cox proportional hazard model (method forward) adjusted by sex and age.
Unigenes with NR annotation were further analyzed with Blast2go (http://www.blast2go.org/) to obtain their gene ontology (GO) annotations, and were then further classified according to GO functions using the Web Gene Ontology (WEGO) annotation software.
The interaction between IgG1 4, with or without EndoS treatment, with FcγRs was further analyzed by surface plasmon resonance.
The underlying DNA sequences of the identified peak regions were obtained with a python script and further analyzed with web-based motif-finding tools.
Data were gathered with Data Collection v.3.0 software and further analyzed with GeneMapper ID-X 1.1 software.
miRNAs found to be significantly associated with case control status were further analyzed with ingenuity pathway analysis (IPA) [ 32].
The association of RGS17 variants with SD was further analyzed using the haplotype association analysis approach.
Considering the positive association of SYN2 mutations with ASD, we further analyzed the impact of mutant SYN2 in vitro.
Those with LOH13q were further analyzed for copy-number alterations to determine whether observed LOH was due to large segmental deletion or CN-LOH.
Genes associated with immobilization were further analyzed to reveal pathways and biological significance and validated by immunohistochemistry on sagittal sections of knee joints.
