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Table 4 Neutralization of Plant water (pH 3.2) with filtrate of raw lime suspension Water (mL) Normality (N) Base required (mL) pH Amount of neutralizing base (g/m3) 100.0 0.1 2.8 5.2 39.2 100.0 0.1 3.0 6.1 42.0 100.0 0.1 3.1 7.2 43.4 (iii) Neutralization experiments with calcium carbonate (CaCO3) .
Controls included plants not incubated with E. coli and plants incubated with filtrate of 15N-E.
rIDE increased virus infectivity when incubated with cell-free ROka-lacZ for 1 or 3 hr at 37°C compared with buffer or with filtrate passing through the rIDE purification (Fig. 2C).
For inorganic nutrient analysis, samples were filtered through 0.2 µm Nuclepore Track-Etch membrane filters (Whatman) into HDPE scintillation vials with cone-shaped plastic lined lids (Fisher Scientific), after rinsing both the bottles and lids three times with filtrate.
Fifteen ml of filtrate was collected in HDPE scintillation vials with cone-shaped plastic lined lids (Fisher Scientific) after rinsing both the bottles and lids 3 times with filtrate, and then stored at −20°C.
We conclude from these experiments that planarian protonephridia, like vertebrate nephrons, combine ultrafiltration with filtrate modification in the same structure.
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Each core was evacuated for 12 h and saturated with filtrated actual formation brine.
The transforming ability was evaluated by transformation of competent cells with filtrates of E. coli pEGFP cultures.
Numerical examples are presented, including flow past a system of bodies in a channel, flows in branched channels with or without particles, and in channels of arbitrary shape with filtrating walls.
Arrays were washed twice at 42°C with 2× SSC +0.2% SDS, and twice at 23°C with 2× SSC, followed by more stringent washes at 23°C with 0.2× SSC and with filtrated H2O.
Cells treated with filtrates from Trichoderma wild type grown alone showed the prevalence of HO positive/PI negative nuclei, indicative of early PCD-like stages, characterized by chromatin condensation and an intact plasma membrane (Fig. 5c).
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