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The total R5 HIV-1 replication over 18 days in the presence of both compounds (added repeatedly with every medium change) was 54.7%±8.3 (mean ± SEM) (p∼0.01, n = 5) higher than in matched tissue blocks treated with the agonist only, although the level of replication was not restored to the level of the untreated control.
Fresh AZA was added with every medium change and supplemented with 300 nM Trichostatin A (Sigma-Aldrich) for the last 16 h of culture.
This results in an accumulation of unprocessed procollagen in the cell culture medium [ 73] where it does not belong and is discarded with every medium change.
For experiments with addition of MIF to culture media, recombinant human MIF was added at 10 ng/ml and 50 ng/ml with every medium change.
The test agents were added concurrently with the start of NGF treatment, and cultures were maintained for 6 days, with the indicated agents included with every medium change.
The test agents were added concurrently with the start of NGF treatment, and cultures were maintained for up to 6 days, with the indicated agents included with every medium change (48-hr intervals).
Similar(53)
They seem to challenge themselves with almost every medium imaginable, ranging from disciplined painting studies to video art, performances and social experiments.
He began as a graphic designer but has since experimented with nearly every medium, from painting, to illustration, sculpture, and digital graphics.
The group's goal, he said, is to "hit every medium with the same message and go deeper".
Artists all over the world experimented with every conceivable medium.
This method ensured the preservation of the conditioned medium with secreted autocrine factors at every medium change.
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