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Bone marrow cells from CD45.2+ IκBα-SR Tg mice were mixed with equal cell numbers from CD45.1+ wild-type mice and intravenously injected into lethally irradiated Rag1−/− mice.
For alloactivation, mixed lymphocytes culture reaction was performed using donor cells which were inactivated and incubated with equal cell number of recipient lymphocytes (200.000 total cells/well) for 72 h at 5% CO2, at 37°C.
For cell growth analysis, the stable cells were plated in a 24-well plate with equal cell density.
The studies were conducted with equal cell seeding density to avoid any confluences-related difference in effect observed on cell viability.
This would agree with the structure for Bi1– x Sr x MnO3 with equal cell volume as our BSMO films (58.8 Å).
The cell doubling rate was measured during passages 1 to 8 as described above by counting the cells each day (parallel experiments with equal cell numbers per well as described in methods).
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Serum bottles with 25 mL GS2 medium and 150 g/L α-cellulose were inoculated with approximately equal cell concentration (about 10 CFU/mL) of C. phytofermentans and S. cerevisiae cdt-1.
The evaluation of the total metabolic active cell count and the individual metabolic active cell counts starting with an equal cell numbers of different species present in a mixed culture offers a quantification which would be helpful in designing, operating and controlling processes using mixed cultures.
Duplicate flasks were pooled to provide sufficient starter material, and divided into four aliquots with an equal cell concentration.
Although control divisions gave two daughter cells with equal apical cell surfaces, divisions in Noc + LatB-treated embryos were unequal with one daughter cell having a much smaller apical cell surface than the other.
In this work, the freshly isolated oral cavity cancer cells with equal total cell number (5.7 × 10) were cultured in 3 different culture models (i.e., 2D, 3D, and spheroid cell culture models) and under different medium pH (pH 6.6, 6.8, 7.0, 7.2, and 7.4) conditions using the perfusion microbioreactor system).
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