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The proteins in the gels are then visualised with each resulting spot ideally representing a single protein.
Over the course of 3 months prednisone tapering was attempted three additional times, with each resulting in symptom recurrence.
These ATPalpha CJ mutants all exhibit reduced respiration consistent with each resulting in a loss of ATPase ion-transport function.
We searched for less complex models that might have performed equally well, by dropping each of the 13 terms one-at-a-time, and evaluating the mean concordance associated with each resulting 12-variable model.
Briefly, DNAs from toxins displayed on the phage were inserted between SpeI and SacI sites of GST (glutathione transferase -Cry1Aa protransferase -Cry1Aan exprotoxin vector, pB9 and E. coli BL21 was transformed with each resulting vector [ 31].
Both regions were connected by overlap PCR and resulting DNA fragments were digested by restriction enzymes and then inserted between SpeI and SacI sites of GST-Cry1Aa protoxin fusion-protein-expressing vector, pB9, and E. coli BL21 was transformed with each resulting vector.
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Heterozygous mice with the targeted allele (Ing2flox−neo/+) were crossed with each other, resulting in homozygous mice (Ing2flox−neo/flox−neo).
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As a result, the plasma species experience many collisions with each other, resulting in the formation of larger droplets.
These fibrils connected with each other, resulting in a network formation (Fig. 2C).
Data were permuted 1000 times with each permutation resulting in a calculated t-statistic.
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