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The difference in representation between known open and closed chromatin loci can be reliably detected by the GCSDI assay after treatment with diverse amounts of DNase I, and thus is a reliable analysis outcome that is not overly sensitive to DNase I treatment conditions (Additional file 1: Figure S1).
DNase I (Promega) digestion was performed at 37°C for 10 or 15 minutes with diverse amounts of the enzyme to optimize the procedure (0.1U, 0.5U, 1U, 2U and 5U); for further analysis cells were treated with 0.5U DNase I for 10 minutes.
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In order to investigate potential interferences on the recovery of silver ions, a 5.0-mL aliquot solution containing 0.5 μg L−1 silver ions with various amounts of diverse ions was treated according to the recommended procedure.
Experiment with various amounts of products.
There is a large and diverse amount of information associated with the more complex task of walking with object transport, leading to an increased demand for processing and greater cognitive or attentional load [ 30].
There is a "pretty diverse" amount of that going on, according to Mr Holden.
All patients are different, so what is needed is a diverse amount of products on offer.
The vast and diverse amount of information available can result in information overflow.
Join a diverse amount of groups.
In order to produce higher amount of flavonoids, several recombinant microorganisms have been constructed with diverse combination of flavonoid biosynthesis genes.
It is a disorder with diverse symptoms.
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