Anyway, Mr. Penn's photographs follow this trend, the artist approaching each wad like a crime scene: he snaps the evidence with detached, scientific scrutiny.
With detached, rueful laughter, Albee conjures up a camp nightmare about grief, a demonstration of what psychiatrists call "negative hallucination" — Homo sapiens's inability to see what is right under his nose.
With detached compassion, your doctor will give you nine years to live.
When cells reached 70 80% confluence, they were washed with PBS, detached with 0.25% trypsin, and fixed with 75% ethanol overnight.
Cells were washed twice with PBS, detached with 100 μL accutase (PAA; Pasching, Austria) and transferred into flow cytometry tubes.
Cells were washed with PBS, detached with 0.25% trypsin-EDTA (Gibco), then split at ratios from 1 2 to 1 5.
Thereafter cells were washed twice with PBS, detached with 0.02% Pronase in PBS and collected by centrifugation (1400 rpm).
After the treatments, cells were washed twice with PBS, detached with a standard trypsinisation procedure and resuspended in PBS.
Cells were then washed with PBS, detached with 0.25 % trypsin and centrifuged in DMEM at 200 g for 5 min.
For total cell extracts, adherent cancer cells were washed once with PBS, detached with trypsin, and collected in the culture medium.
