Sentence examples for with corresponding restriction from inspiring English sources
*Restriction sites with corresponding restriction enzyme are represented in italics.
To generate pBUH3 and pBUH4 binary vector plasmids, Bam HI and Hin dIII sites were removed from a binary vector plasmid pBCH1 (Ito et al.[2001]) by cutting these sites with corresponding restriction enzymes and re-ligation after filling-in.
PCR products were purified, digested with corresponding restriction enzyme(s), and ligated to pHsh at the respective Stu I/Xho I sites, to generate pHsh-xarB.
The products were excised with corresponding restriction endonucleases (New England BioLabs) and cloned into the pCS2+ vector between the EcoR I-Xho I sites.
The PCR-amplified NA and M1 encoding DNA fragments were digested with corresponding restriction enzymes and cloned into the pFastBac plasmid under the polyhedron promoter, and confirmed by DNA sequencing.
Resulting PCR products were digested with corresponding restriction enzymes and ligated into the vectors pProEx-HTc (Invitrogen) and/or pGEX-5X-3 (GE Healthcare Bio-Sciences) previously digested with the same enzymes.
The sequences are subdivided into libraries, which are labeled with a three-letter code, with the corresponding restriction enzyme listed between brackets.
All PCR products were ligated into pGEM T-easy vectors and digested with their corresponding restriction endonucleases (Fig. 4b), before fusion with the hrGFP vector backbone.
The PCR product was purified and digested with HindIIIand Xho, then cloned into a mammalian expression vector pcDNA3.1 with the corresponding restriction sites (Novagen, Darmstad, Germany).
After digestion with the corresponding restriction enzymes, the DNA fragments were cloned into a pUC18 vector to generate the recombinant plasmid pUC ga5dhLR.
After digestion with the corresponding restriction endonucleases (NEB or Fermentas, Beijing, China), segment was ligated into pET-28a to construct the carrier plasmid pX12345LT.
