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The test simulates the behavior of the waste by contacting it with an acidic media during 18 h with continuous agitation.
The suspensions were then heated at 90°C for 1h with continuous agitation (200 rotations/min).
The resulting slurry was aged for 18 h with continuous agitation.
The broth was fermented at 32 °C for 72 h in an automatic incubator with continuous agitation (150 rpm).
Fresh medium was inoculated in test tubes with the starter culture and grown at 35.5°C with continuous agitation at 30 rpm.
The sucrose solution (20 g in 100 mL of water) was heated at 90°C for 1 h with continuous agitation (200 rotations/min), and 200 mL of NaOH (2 mol·L−1) solution was added slowly.
Similar(19)
Ramnolipids were extracted with diethyl ether under continuous agitation for 12 h.
Membranes were incubated with primary antibodies for 16 hours at 4°C under continuous agitation, washed three times with TBS-T, and incubated with secondary antibodies for one hour at room temperature.
Supernatants were then incubated with 100 200 μl of Glutathione-sepharose resin (Amersham Pharmacia) pre-washed with PBS (pH 7.4), under continuous agitation at room temperature.
Each reactor is equipped with a magnetic stirrer for the continuous agitation of the test sample.
The resultant material, referred to as bovine decellularized matrix (bECM) was rinsed in PBS supplemented with 1% w/v penicillin/streptomycin under continuous agitation for 24 h at 4 °C to remove residual cellular material.
More suggestions(15)
with continuous thrust
with continuous music
with continuous improvement
with continuous administration
with continuous transesophageal
with continuous infusion
with dull agitation
with continuous location
with continuous vancomycin
with continuous growth
with continuous outcome
with mild agitation
with continuous enrollment
with continuous news
with continuous surveillance
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com