Sentence examples for with continued passage from inspiring English sources

Consolidation is defined as the process, by which motor memories become increasingly stable with continued passage of time, and one mechanism of interference is disruption of consolidation [5].

We considered that selection pressure might result in the loss of caspase-8 DEDs, and increased proliferation and tumor formation might result with continued passage of the cells.

Consolidation may be defined as a set of processes whereby a motor memory is stabilized with continued passage of time and becomes less susceptible to disruption from a competing memory [19], [20].

It is clear however that with continued passage in serum-free conditions, even the three predominant protein bands will eventually disappear or remain barely visible by Coomassie blue staining, which may explain why Raoult et al. [19] had to use a much more sensitive dye (silver nitrate) to visualize these same protein bands in serum-free conditions.

Several studies have shown that the loss of multi-potency with continued passage is not associated with changes in MSC phenotype [ 51, 52].

Indeed, like other types of progeroid cells, HGPS and RD cells accumulate DNA damage, in particular DSBs, with continued passage in culture [ 12, 18, 19], indicating that DNA repair activity is impaired in these cells.

However, with continued passaging of ASCs, these APC-associated markers are lost, effectively mitigating the immune response until it has been eliminated.

Some of these immortal cultures eventually do reveal loss of the WT allele with continued passaging, but others retain the original unmutated p53 allele.

The reasons for the loss of sensitivity that occurred with continued passaging in the present experiments are unclear, as these cells had presumably been extensively passaged prior to their acquisition for the study reported here.

When culture dishes became near confluent, cells were dissociated with 0.025% trypsin-EDTA (Gibco) and suspended for continued passage.

Adherent colonies were detached by treatment with D-PBS based TryPLE Express (Invitrogen) containing a trypsin-like enzyme and 1 mM EDTA for 5 min at 37°C, and subsequently re-plated for continued passage 1 2.