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We also grew cells on inserts at unit gravity with constant mixing.
The reaction mixtures were incubated for 2 hours at 22°C with constant mixing on a magnetic stir-plate.
In the case of a mixture with constant mixing proportions, the weight of each component will be very close to zero.
The sample was allowed to react for 2 h with constant mixing at room temperature, and three final washes were performed using the 20 mM Tris HCl, 5 mM MgCl2 at pH 8.0.
After carefully removed the supernatant, 0.75 mL of permeabilization buffer was added to the cell pellet, vortex shortly, and then incubates 10 min at 4 °C with constant mixing.
The blocked blot was exposed to the appropriate primary antibody in blocking buffer with constant mixing.
Similar(16)
Carbon dioxide (CO2) is set to 395 p.p.m. with a constant mixing ratio with height and mixing ratios of O3, N2O, CH4, CO and O2 are set to values from the standard atmosphere.
On the Caribbean side, the seasons are less defined, with a constant mix of rain and sunshine.
With globalization, migration and the constant mixing of cultures and creeds, our world is becoming less homogeneous and siloed by the day.
The cuvette-holder was equipped with a magnetic stirrer allowing constant mixing of the sample and a circulating water bath for temperature control.
Each murine monoclonal antibody (0.5 µg in 10 µl PBS 7.4) was added to a 100 µl aliquot of whole blood anticoagulated with sodium citrate and let stand at ambient temperature for 60 minutes with constant, gentle mixing.
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