Exact(8)
Briefly, the cells were stained with cocktail of AO (100 μg/ml) and EB (100 μg/ml) in RPMI 1640 medium at 37°C for 10 min, then the cells were washed with PBS twice.
Cells or tissues were washed with phosphate buffered saline (PBS) and lysed in RIPA buffer with cocktail of protease inhibitors (Roche).
Briefly, spleen cells were incubated with cocktail of biotin-conjugated antibodies against antibodies (CD90, CD45R, CD49b), followed by incubation with anti-biotin microbeads to deplete of T, B and NK cells.
Prior to incubation with LysoTracker Red, RAW 264.7 macrophages (5×105 cells per well) were incubated with cocktail of endocytosis inhibitors containing a mixture of nystatin (25 µg/ml), genistein (200 µM), chlorpromazine (6 µg/ml) and brefeldin A (10 µg/ml) for 30 min as described [40].
Total proteins were extracted from the half of brain by homogenization in RIPA buffer with cocktail of protease inhibitors (Roche).
MSCs trans-differentiated into cardiomyocytes with cocktail of growth factors [ 84] were used to treat myocardial infarction and heart failure secondary to left ventricular injury [ 147].
Similar(52)
This is the reason that modern medicine treats bacterial diseases with cocktails of antibiotics.
The World Health Organization now endorses first-line treatment only with cocktails of artemisinin and at least one other drug.
And drug addicts like Pargat Singh are crouched in the shadows, injecting themselves with cocktails of synthetic drugs.
Fracking involves thousands of gallons of water being mixed with cocktails of chemicals and sand and pumped deep into the earth to break up gas-rich shale rock formations.
It is only a matter of time, it seems, before people will spray their bodies with cocktails of friendly bacteria designed to heal cracked skin, soothe itchy scalps, prevent foul-smelling underarms and stinky feet.
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