A quartz substrate which is a stable material at harsh environment was etched isotropically with buffered oxide etchant (BOE).
After fixation, the samples were washed in 0.1-M cacodylate buffer and post-fixed with buffered osmium tetroxide for 30 min.
Five fish from each aquarium were anesthetized with buffered tricaine methanesulfonate (20 mg/L), and blood was collected from the caudal vasculature.
Two fish from each aquarium were anesthetized with buffered tricaine methanesulfonate (30 mg/L), and blood was collected from the caudal vasculature in Eppendorf tubes containing 500 U of sodium heparinate/mL, used as an anticoagulant.
Abbreviations: Sham unstretched cells under normocapnia, Normo high cyclical stretch with normocapnia, HCA high cyclical stretch with hypercapnia, MA high cyclical stretch with metabolic acidosis, BHC high cyclical stretch with buffered hypercapnia.
Anesthetized mice were perfused transcardially with buffered fixative containing paraformaldehyde, L-lysine, and sodium m-periodate [60].
Soil phosphatase activity was determined by measuring the p-nitrophenol (PNP) released by phosphatase when soils were incubated with buffered (pH 7.0) sodium p-nitrophenyl phosphate solution and toluene at 37°C for 24 h [34].
and perfused transcardially with buffered saline followed by 4% paraformaldehyde in saline.
Red blood cells were lysed by incubation with buffered ammonium chloride solution.
