Your English writing platform
Discover LudwigExact(59)
One must be aware that NPs may interact unfavourably with assay components.
Total protein (50 µg) was mixed with assay buffer containing 20 μmol/L Ac-DEVD-afc substrate.
Structural arguments in conjunction with assay data explain why targeting this pocket is also advantageous for selective binding to Chk1.
The velogenic and lentogenic amplifications were highly reproducible with assay variability 0.45 ± 0.31% and 1.30 ± 0.65%, respectively.
Furthermore, nanoparticles with assay reagents in the absence of cells were also analysed, and no significant effect on absorbance, fluorescence or luminescent readout was observed (data not shown).
Cells were harvested and stained with assay reagents and cell apoptosis was determined by flow cytometry using a FACS Caliber (Becton Dickinson, CA, USA).
Taqman(R) Gene Expression Assay (Applied Biosystems) was purchased for Mash1 with Assay ID Hs00269932-m1.
High-throughput assays were performed using 96-well plates with assay volumes adjusted to 100 µL.
Other notable contributors who provided assistance with assay development and interpretation include Candace Mathiason and Timothy Kurt.
The measurement range was 0.43 110 nmol/L with assay repeatability values of 5% (within series) and 8% (between series).
Briefly, 96 well plates were coated with capture antibody overnight at 4°C and then blocked with assay diluent.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com