Optimal Tm is thought to be ~39 °C, with an upper threshold of 43 °C, above which there are impairments to muscle function (Åstrand and Rodahl 1986; McRae and Esrick 1993).
Lastly, additional markers were added to the MLE-consensus maps using the program bin_script, with an upper threshold of 0.1, a lower threshold of 0.0, an upper gap threshold of 3, and a lower gap threshold of 1 to allow markers that map to identical positions to be put into a common bin.
This was done in accordance with other studies, because higher concentrations have been shown to be associated with drug-related toxicity [ 23, 26, 34], whereas Pea at al. showed that in long-term treatment with linezolid an upper threshold of the target concentration range of 7 mg/L should be favored [ 49].
With 2 peptides at an upper threshold of 5,000 Da (Table 3), several putative proteins are not detected, including Q3HM31_SOYBN Hydrophobic seed protein and Q692Y3_SOYBN Glycinin gy1 (Fragment).
With 3 peptides at an upper threshold of 5,000 Da (Table 3), the not detected protein list is mainly composed of putative and uncharacterized proteins and other protein fragments that have short amino acid sequences.
Muscle CSA was determined by se-lecting the area with a lower threshold of 40 mg/cm3 and an upper threshold of 280 mg/cm3 HA density after smoothing the image (con-tour mode 3 and peel mode 1, and contour mode 1 and peel mode 2 for subtracting the bone area).
In general, larger penises were preferred but with diminishing returns as penis size increased, suggesting an upper threshold of desirability.
In total, 100 μl of supernatant was then diluted in Isoton II and the platelets were counted using a 50 or 70 μm aperture tube with a lower threshold of 1.4 fl and an upper threshold of 24.4 fl.
Thus, we used an upper threshold of 255, which captures the densest bone.
For the signal intensity data generated by one-channel oligonucleotide microarrays, Affymetrix's GeneChip, we applied a lower threshold of 20U and a upper threshold of 16,000U.
An upper threshold of 255 and a lower threshold of 75 were used to delineate each pixel as bone or non-bone; simple global thresholding methods were used.
