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Measurements were performed in phosphate buffer pH 6.0 with an applied potential of +50 mV vs Ag/AgCl and a flow rate of 100 μL/min.
The experiments were performed at 20 °C, with an applied potential of −1.0 V/saturated calomel electrode (SCE), in artificial seawater-like solutions with various SO42− concentrations.
With Pt-MWCNT nanohybrids surface modification, current generated in response to H2O2 by the screen-printed carbon electrode strip was enhanced 100 fold with an applied potential of 300 mV.
A linear response range for NADH between 1.0×10−5 and 5.2×10−4 mol l−1, at pH 7.0, was observed for the electrode, with an applied potential of −200 mV versus SCE.
The optimized conditions for the sensor response were obtained in 0.25 mol dm−3 Pipes buffer (pH 7.0) containing 150 μmol dm−3 H2O2, with an applied potential of −50 mV versus saturated calomel electrode (SCE).
A linear response range for NADH between 4.0×10−5 and 8.0×10−4 mol l−1, at pH 7.0, was observed for the electrode, with an applied potential of −50 mV vs. SCE.
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The highest specific surface area value (1315 m2 g−1) was obtained with carbon powder deposited at 450 °C for an applied potential of −6 V and dried at 600 °C under vacuum after washing.
For example, the surface condition and composition of the nanowires varied with electro-deposition time at 60°C under an applied potential of 1.6 V [47].
PB-modified SPEs with a 10% PB loading, operated at an applied potential of −0.05 V, were thus used for all subsequent chronoamperometric measurements.
The electrode based on fifth generation of MWCNT-PAMAM and urease enzyme showed excellent performance in urea detection at an applied potential of 0.45 V with short response time of 3 s, wide linear range of 1 20 mM, detection limit of 0.4 mM, and sensitivity of 6.6 nA/mM.
The proposed NADH biosensor exhibited a wide linear response range of 1 μmM13 mM during amperometric testing using an applied potential of 0.42 V, with a low detection limit of 0.18 μM (S/N = 3).
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