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Empirical estimates of the number of mutation and recombination events contributing to the diversification of SLVs were made as previously described [21], [44], with additional modifications.
The genomic DNA derived RNAi library was constructed with additional modifications to prevent self-ligation of vectors and fragments [ 20].
Data were pre-processed using the pipeline described in Touleimat and Tost [ 21], with additional modifications [ 21].
The cycle of independent analysis and review was repeated with additional modifications to correct any remaining errors followed by a final full run.
The remaining peptides from each sample were subsequently enriched for phosphopeptides using TiO2 chromatography as previously described [ 70] with additional modifications.
Such probes represent useful tools for O2 sensing and particularly for real-time monitoring of icO2, which can be realized on existing commercial TR-F readers or, with additional modifications, in ratiometric intensity-based detection formats.
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The materials used for this fabrication are potentially biologically inert and, with some additional modifications to the design, can be chronically implanted with minimal side effects.
We therefore re-ran Model 1, with two additional modifications (Model 2: see Methods and Analyses).
We used HCUP NIS data for 2010 for the comparison with no additional modifications.
Primers were designed based on criteria stated by Wojdacz et al. [ 9] with some additional modifications using the Methyl Primer Express v1.0 Software Appliedd Biosystem, USA).
In brief, questions about physical activity were adapted from validated questionnaires used in several other studies in Western [ 18] and Chinese populations [ 19], with some additional modifications following a CKB pilot study.
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