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Dried samples were dissolved in 1 ml of methanol 80% with add of vortex and sonication, and filtered using 0.45 µm Nylon (CostarR) filters held in a 2.0 ml polypropylene tube and centrifuged at 5000 rpm for 45 sec before analysis.
Moreover, isomiR for miR-21, with ADD of cytosine on 3' end, had the highest number of reads (averaged for all libraries) among all detected isomiRs.
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Furthermore, the optimum agitation time can be reduced by 88% with adding of branch chain polyols pentaerythritol.
The PS particle size is greatly reduced with adding of SBS, and the reduced particle size results in greater resistance to deformation, which causes the co-continuous structure at oriented zone change into droplet morphology.
The carried out investigations of ZnS Cu obtained by SHS method with adding of NaCl in charge as flux have shown, that introduction of NaCl enables to increase quantity of fine fraction (50 500 nm) in material.
As a consequence, an improved impedance model with the add of a diffusive element is proposed in this paper.
His circle included perhaps 30 men, most of them Sicilians, with added groups of Tuscans and southern Italians.
Whereas the likelihood ratio usually increases with the adding of variables to a risk score, the AIC punishes adding variables that do not substantially increase model fit.
Red wine also adds richness, and I've also experimented with adding shot of vodka at the end.
As a game, it should make for an enthralling spectacle, with added layers of meaning for extra resonance.
We'll be able to interact with the content, with added layers of engagement.
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