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The Obama administration has given GM until the beginning of June to come up with a viability plan to avoid following its smaller rival Chrysler into bankruptcy.
Some types of cells, such as human umbilical vein endothelial cells (ECV304), proliferated with a viability rate similar to those on the polystyrene surface (Fig. 7c).
The in vitro cytotoxicity results revealed insignificant toxicity effect towards normal cell line, with a viability of > 80% even at higher concentration of 50 μg/mL.
But according to a government-led task force appointed to come up with a viability plan for the airline, JAL now has negative net worth of as much as ¥800 billion.
In addition, cell viability higher than 90% was detected after embedding cells in the MC-based hydrogel; moreover, the optimized printing parameters allowed to bioprint C2C12 cells embedded in the MC-based hydrogel with a viability higher than 80%.
The cells are then incubated with a viability dye (Molecular Probes, Invitrogen, Eugene, OR, USA).
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Cell suspensions were first stained with a fixable viability dye (Fixable Viability Dye eFluor 506, eBioscience) for discrimination of live and dead cells.
The microbial cell viability assay was performed with a Microbial Viability Assay Kit-WST (Dojindo, Japan).
Viability of cells was analyzed with a cell viability kit (BD Biosciences, CA, USA) on a flow cytometer as described previously [ 18].
While cells with GSH alone had a viability of 72% after 72 hrs, isofuranonaphthoquinone-treated cells produced 37% viability.
When sperm from the male with a high viability ejaculate were combined with the seminal fluid from his rival with a relatively low viability ejaculate, the viability of his sperm was unaffected; the slope did not differ from 1.0 (1.14±0.32, t = 0.45, df 19, P = 0.658) and the intercept did not differ from zero (−0.19±0.26, t = 0.71, P = 0.484) (closed symbols in Fig. 3).
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