A soluble form of the murine class II MHC molecule, I-Ad, with a leucine zipper tail added to each chain to enhance dimer assembly and secretion, has been produced in Drosophila melanogaster SC2 cells.
From 1970 to the 1977 78 season, the team wore red road uniforms, switching to black in that year, with a switch from horizontal lettering with the tail added on the last letter to vertical lettering midway through its lifespan.
For 5′RACE, RNA was transcribed with gene specific primers and a homopolymeric tail added to the 3′ end of the resulting cDNA.
is a chain complex of C0/∂1C1-modules and a quasi 3-crossed complex is just a quasi 3-crossed module with a chain complex as tail added on.
We designed a universal-forward primer with an M13-tail added at the 5' end (M13BC-FW 5'-TGT AAA ACG ACG GCC AGT-HAA YCA YAA RGA YAT YGG NAC-3'), similar to other primers previously used in other barcode approaches [e.g. 13].
The primers were commercially synthesized (Sigma-Aldrich, Dorset, United Kingdom) with forward primers having an M13-tail added to their 5′ end (5′-CACGACGTTGTAAAACGAC-3′).
Sizes of the DNA fragments and their exon-intron structures (boundaries) are given in additional file 2. Polymerase chain reactions (PCR) and cloning were performed using the mark-recapture (MR) method developed by Bierne et al. [ 33] with 4-nucleotide tails added to the 5' end of each primer.
hen add a tail, and add hooves to the back legs.
The ClpXP and ClpAP proteases degrade proteins with carboxy-terminal peptide tails added by the SsrA-tagging system.
Gottesman, S., Roche, E., Zhou, Y. & Sauer, R. T. The ClpXP and ClpAP proteases degrade proteins with carboxy-terminal peptide tails added by the SsrA-tagging system.
The forward primer for each primer pair was synthesized with an additional modified 20-bp M13 tailed added to the 5′end.
