The rGO/PP composites were prepared through a latex technique, which consists of an in-situ chemical reduction of graphene oxide in PP latex and a subsequent filtration.
One ml of plasma was centrifuged at 5,000×g at 4°C for 5 min with subsequent filtration of the supernatant through a 0.45-µm filter (Millipore, Billerica, MA, USA) to remove residual host cells.
Aqueous solution of sodium hypochlorite was prepared by dissolution of calcium hypochlorite and sodium hydroxide in water, with subsequent filtration of the calcium hydroxide precipitate formed.
During mycobacterial infection, LAM is released from metabolically active or degrading mycobacterial cells into the blood stream with subsequent filtration by the kidneys, passing into urine [ 6, 7].
It has been postulated that LAM is released from metabolically active or degrading mycobacterial organisms into the serum, with subsequent filtration by the kidneys, passing into the urine where it can be detected by enzyme-linked immunosorbent assay (ELISA) [ 14].
A sample preparation was only based on extraction with methanol water mixture and subsequent filtration of the extract.
Steroid-free suction blister fluid was generated by stirring suction blister fluid from different volunteers two times with active charcoal and subsequent filtration.
Metal-free buffers were obtained by treatment with Chelex-100 resin (BioRad, Hercules, CA) and subsequent filtration through a 0.22-μm filter.
7 Since it is performed through a clear-corneal incision, subsequent filtration surgery is not affected.
DNA was purified using a silica-based purification kit (Geneclean kit, Q.BIOgene, USA), which provided a faster, easily scalable and equally efficient method compared to the previously described precipitation with trichloroacetic acid and ethanol and subsequent filtration through GF/C filters.
