Sentence examples for with a stringency from inspiring English sources

However, in all cases, the selection of genes undergoing expression variation is associated with a stringency parameter.

Next, to confirm these results and to determine relevant target genes in these pathways, we repeated the analysis with a stringency of 1 median false positive per dataset (implicating essentially all detected genes to be disease related) to increase confidence that pathways were disease related.

P ≤ 0.01 was considered significant, with a stringency of ± 1.5-fold difference in expression levels.

To this end, we performed an iterative read-mapping starting with a stringency of 0 and ending with 3 mismatches.

Whole genome sequence comparisons were performed using CoreGenes with a stringency setting of 75 http://www.binf.gmu.edu/genometools.html[ 96, 34].

The TargetScan algorithm was used to predict the biological targets of miRNAs with a stringency of 0.7.

Prediction of the CC domain was conducted using the COIL program (http://embnet.vital-it.ch/software/COILS_form.html) with default settings and a stringency of 90% threshold.

The next day, the membrane was washed twice with a high stringency solution (2X SSPE and 0.1% SDS), and twice with a low stringency solution (0.2X SSPE and 0.1% SDS) for 15 minutes each at room temperature.

In Mu transposition, the target DNA sites are selected with a low stringency of sequence specificity [15], yielding essentially random distribution of integrations along longer regions of DNA [14], [16].

A target prediction algorithm with a scoring stringency similar to those previously used for miRNA target prediction was applied in this procedure (a host gene with a score of 3.5 or lower for a specific viral siRNA was considered as a potential target; see materials and methods) [26].

Given the early availability of both the human and mouse genomes, initial efforts focused on human mouse pair-wise comparisons; but subsequent studies frequently used more distant comparisons such as human-fish to uncover functional non-coding elements with a higher stringency [15][16].