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Cellulosic substrates by hot-washing process were added to a 500 mL round-bottomed flask with a stir and diluted with acetate buffer solution, to get a final solid concentration of 250 g per liter.
When preparing the nanofluid, the gum arabic at a concentration of 0.25 wt% was first dispersed into the ethylene glycol in a 500-ml glass breaker, which was placed on a stirrer with a stir bar rotating inside the fluid; after the gum arabic was fully dissolved into the ethylene glycol, 0.5 wt% MWCNTs were dispersed into the fluid.
A microwave reaction vessel was equipped with a stir bar and charged with diketone 19 (44 mg, 0.2 mmol), NaOAc (20 mg, 0.24 mmol) and NH4OAc (54 mg, 0.7 mmol) in 1 mL of EtOH, the resulting mixture in the reaction vessel was sealed and allowed to stir for 5 min at rt, then heated by microwaves at 40 °C for 12 min in a CEM-microwave reactor.
This factor was determined using the calibration procedure stated in the Unisense manual: a 2-ml sample of LBK was sparged with 20% H2/N2 and assayed at 37°C with a stir bar (200 rpm).
Absorbance was monitored at 734 nm on a UV-visible spectrophotometer until stable (<10 sec), and then 20 mL (for HAA) or 40 mL (for LAA) extract was added, mixed with a stir paddle, and monitored at 734 nm until absorbance reached a minimum.
The IL-17 receptor complex interacts with a STIR (SEFIR, similar expression to fibroblast growth factor genes) subdomain leading to activation of act1, TRAF6, nuclear factor kappa B (NFκB), activator protein 1 (AP-1) and mitogen protein kinases (MAPK) [10], [11], [12], [13], [14].
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Each individual clonemate was settled into a labeled, 125-ml glass chamber with a stir-bar cage for the duration of the experimental period to minimize disturbance and the risk of damage prior to respiration measurements.
A well-marginated region of fluid-like signal (i.e. hyperintense with a STIR-w sequence and hypointense with a T1-w sequence) was diagnosed as an intraosseous ganglion when located within bone, or as an extraosseous ganglion when located in soft tissue.
Fluorescence spectra were measured in a Spex Fluoromax spectrofluorimeter equipped with a stirred cell compartment.
The yeast broth (4 L) was percolated through filter paper (Toyo Roshi Kaisha, Japan) and concentrated with a stirred ultrafiltration cell (model 8400; Millipore Corp).
Peak fractions were pooled, concentrated with a stirred cell concentrator (EMD Millipore, Bilerica, MA) and dialyzed exhaustively against HEPES, pH 7.4, 150 mM NaCl.
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