Sentence examples for with a single guide from inspiring English sources

But Tayler would make his descent with a single guide.

The template is triisopropylsiloxy, where the geometry about the oxygen is drawn in a linear fashion, rather than the more commonly used bent orientation, due to congestion. Figure 12 Grafting of a template with a single guide atom.

Here, we report the crystal structures of SaCas9 in complex with a single guide RNA (sgRNA) and its double-stranded DNA targets, containing the 5′-TTGAAT-3′ PAM and the 5′-TTGGGT-3′ PAM, at 2.6 and 2.7 Å resolutions, respectively.

With this approach, termed CRISPR interference (CRISPRi), transcription of a specific target sequence is repressed by a catalytically inactive Cas9 protein recruited to the target DNA by base-pair interactions with a single guide RNA that is complementary to the target sequence.

This suggests that CD29 evolves from the snoRNA with a single guide function.

CRISPR-associated (Cas) genes together with a single guide RNA (sgRNA) enable sequence-specific cleavage and editing of DNA.

Figure 13 Grafting of a template by matching a bond with a template atom connected to a single guide atom.

Monorail systems are an AGT concept using a single guide and support beam, usually elevated, with a vehicle riding on top of, or suspended beneath, the beam.

Here, we present the high-resolution crystal structures of the three SpCas9 variants in complexes with a single-guide RNA and its altered PAM-containing, partially double-stranded DNA targets.

Cas9, a type II CRISPR-Cas effector complex, can be programed with a single-guide RNA that base pairs with the target strand of dsDNA, displacing the non-target strand to create an R-loop, where the HNH and the RuvC nuclease domains cleave opposing strands.

To fuse GFP TEV::S (Cheeseman and Desai 2005) at the N terminus of endogenous LIN-41, we targeted a specific site near the initiator methionine for Cas9 scission [protospacer-associated motif (PAM) site at position 9341872 in the genome] with a single-guide RNA (sgRNA).