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Interestingly, with this latter diet, ruminal pH did not change compared with a single addition of starch.
This replaces several floating point adds/multiplies in the inner loop with a single addition operation.
For 5-LOX and FLAP studies, cells were treated with a single addition of 10 μM HNE for 24 hours, 48 hours, and 72 hours in the presence or absence of naproxen (a nonspecific COX-2 inhibitor) or antibody anti-transforming growth factor-beta 1 (TGF-β1).
For 5-LOX and FLAP studies (up to 72 hours), cells were treated with a single addition of 10 μM HNE in the presence or absence of 50 μM naproxen or 100 μg/ml anti-transforming growth factor-beta 1 (TGFβ1).
For COX-2 and mPGES-1 studies (up to 24 hours), chondrocytes were treated with a single addition of 10 μM HNE or with repeated treatments by adding 10 μM HNE to the cultures at 0, 2, 4, 6, 8, 10, 12 and 14 hours.
Secondly, to determine whether TGF-β1 production could be involved in HNE-induced 5-LOX and FLAP expression, chondrocytes were treated or not with a single addition of 10 μM HNE for 72 hours in the presence or absence of 100 μg/ml TGF-β1 antibody.
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The purpose of this study is to explore the cytoprotective effects of the antioxidant Bucida buceras extract in co-treatment with hydrogen peroxide (H2O2) delivery as a single addition or with continuous generation using glucose oxidase (GOx) in ARPE-19 cell cultures.
ARPE-19 cells were exposed to a variety of H2O2 nominal concentrations (400 1200 μM), by delivering oxidant to culture as a single addition (pulse) with serum-free culture medium (Fig. 3b) and with 10%% of serum (Fig. 3c).
However, repeated treatments with HNE prevented the decline of COX-2 and mPGES-1 expression that occurred with a single aldehyde addition.
This observation allows us to conclude that, for the optimal sample amount, all lipid species measured within a lipid class have similar response factors enabling absolute quantification with a single standard addition per lipid class.
For consensus tree calculation, trees were TBR-collapsed, Bremer group supports calculated by TBR-swapping, and bootstrap resampling by 100 replications of symmetric resampling with a single random addition (see Additional file 1).
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