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Median transverse sections (1.0 1.5 mm thickness) of developing caryopses and matured grain were manually cut with a sharp razor.
Developing caryopses at 7 DAF were hand-cut into 1.0 mm-thick sections at the median with a sharp razor and immediately fixed with ice-cold 4.0 % paraformaldehyde and 2.0 % glutaraldehyde for 3 h.
A small piece of a fresh leaf of T. pratense and of the reference standard were chopped together with a sharp razor blade in a Petri dish containing 1 ml ice-cold GPB (general purpose buffer: 0.5 mM spermine × 4 HCl, 30 mM sodium citrate, 20 mM MOPS [MOPS = 4-morpholine propane sulfonate], 80 mM KCl, 20 mM NaCl, 0.5% [v/v] Triton X-100, pH 7.0, according to Loureiro et al. (2007).
Two openings were cut on the head with a sharp razor edge.
From 30-day old plants, leaves were detached with a sharp razor, and gently inserted pair-wise into 0.5 ml semi-solid water with 0.5% agar in a 0.5-ml reaction tube.
To induce fin regeneration, we amputated the caudal fin with a sharp razor blade.
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Fresh leaves of Epidendrum were co-chopped with a fresh leaf of Pisum sativum (internal reference standard with 2C = 8.76 pg) using a sharp razor blade in a Petri dish containing 1 ml WPB (0.5 mM spermine.4HCl, 30 mM sodium citrate.3H2O, 20 mM MOPS, 80 mM KCl, 20 mM NaCl, 0.5% (v/v) Triton X-100, pH adjusted to 7.0).
Plucked hair was placed on clean glass plate and a bulb was cut off with a clean and sharp razor blade under a magnify lens.
Don't pretend like there's a boogeyman in your closet, Or that you are having nightmares about a man with sharp razor claws in your nightmare, Nothing like that ever happens in real life, It's all just made up so it actually makes people think that it's real.
Bulked samples (about 0.5 cm2 for radicles and 1 cm2 for leaf material (per each individual) were sliced by chopping with a sharp clean razor-blade 20-300 chops), in a plastic 9 cm diameter Petri dish containing 1.5 ml of cold (5°C) CyStain® UV Ploidy solution (Partec, Germany) modified by addition of 6.48 mM dithiothreitol (DTT) and 1% (v/v) polyvinylpyrrolidone (PVP-40) (Sigma-Aldrich, USA).
Start with a good, clean, sharp razor.
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