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Messenger RNA (mRNA) sequence data were obtained using either NZGL (www.nzgenomics.co.nz) or Macrogen sequencing facility (www.macrogen.com) on an Illumina HiSeq2000 next generation sequencer, with samples bar-coded 12 to a lane, with a sequencing depth of 17-27 million reads (Additional file 4).
The final effective mapping depth averaged > 54× across the whole genome, with a sequencing depth ranging from 53× in Dongjin to 55× in HY-08.
He and his colleagues also teamed up with a sequencing center--the Whitehead Institute--in a strategy that proved successful for several groups.
Finally, we generated random reads from these chromosomes with a sequencing error of 0.1 1%.
The thylakoids isolated from the wild type or stn8, stn7, stn7stn8 mutant plants were resuspended in 25 mM NH4HCO3, 10 mM NaF to a final concentration of 2.5 mg of chlorophyll/ml and incubated for 3 h at 22°C with a sequencing grade-modified trypsin from Promega (Madison, WI, USA) at 5 µg of enzyme/mg of chlorophyll [22].
This dataset with a sequencing depth of about 10 mio.
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It begins with a sequence written on his final birthday.
His film ends with a sequence of contemporary references to the singer among popular political movements.
The color of the image was adjusted with a sequence of toners, some of them toxic.
St. John's responded with a sequence of scoring chances, including four corner kicks.
(It also bears witness to Lessing's long relationship with a sequence of closely observed cats).
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