Velaglucerase alfa (drug substance lot EP06-001, SHumanHuman Genetic Therapies) was incubated with N-glycosidase F (30 mU/3 μL) for 4 6 h at 37°C with 0.9% NP40, followed by a second addition of N-glycosidase F, and an additional 17 19 h incubation at 37°C.
This study gave a maximum of 45.3% hydrolysis in 24 h, with no improvement observed even after a second addition of cellulase preparation [ 38].
The addition of adsorbed hydrogen atoms is stepwise with the second addition of the adsorbed hydrogen atom being the rate-determining step.
This week, he and journalist Elvis Mitchell -- whom I interviewed in this column last year -- return to HBO with their second addition to the remarkable ongoing documentary, "Black List".
In the IgA statistical modeling, addition of AB was significant both as unique component and in addition with a second agent (AB+EDTA or AB+IP).
In order to ensure a proper behaviour of the adhesive under dynamical, mechanical, thermal or rheological loads, they are typically reinforced with a second phase stiffer material in addition to the adhesive matrix.
We also identified three case reports of patients who had been diagnosed with a second malignant hematologic neoplasm in addition to their PCa.
In addition, with a second locus, each cell count is proportional to the genotype frequencies p k as shown in Table I, and this is also reflected in the "offset" term.
Labionin follows a similar biosynthetic route to lanthionine, with cyclodehydration of a cysteine and two serine residues, which then react in a second Michael addition with another dehydroalanine [ 21– 23].
While sharing some of the conservationists' concerns, Genovesi said that the blacklist could be updated rapidly, with a first round of additions possible next April.
