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With a plating current density above 20 A dm− 2, amorphous Cr-rich alloy deposits were obtained, and crystalline Ni-rich deposits were achieved with an electroplating current density lower than 15 A dm− 2.
The cells were seeded in a Matrigel coated 96-well black plate with a plating volume of 100 μL/well at a density of 3000 15,000/well, and incubated in CO2 incubator to adhere for 12 h before the tested drugs were added.
HUVEC endothelial cells were grown in a special media (199 Media+HAMS F12), and supplemented with 20% heat-inactivated foetal calf serum, 1% Hepes, 1% Glutamine, 12.5 μg Human EGF, 625 ng of Human bFGF, 3750 units of Heparin, penicillin 0.06 g l−1, and streptomycin 0.1 g l−1) Only cultures with a plating efficiency of over 70% were used for analyses.
Most BiFEs have been plated from acetic buffers, although Krolicka et al. used a plating solution of Bi(NO3 3 (0.02 M), LiBr (0.5 M), and HCl (1 M) with a plating potential of −0.25 V for 45 s [addition of bromide and strong acid enabled a highly concentrated Bi III) plating solution to be produced].
For example, when after treatment with 8 Gy X-rays H35 cells with a plating efficiency of 90 100% are seeded at a density of 5000 per flask, 30 clones are formed on average, which means that the surviving fraction is about 0.6%.
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