Sentence examples for with a overhang from inspiring English sources

Overall, our results indicate that RNAs ranging in size from 246 341 nt, ending with a 3' overhang of one nucleotide, and purified using the native affinity technique, could be successfully used in a wide range of biochemical and structural assays.

The Taq DNA polymerase generated PCR products (with A overhang) were ligated directly to pCR 2.1 TA vector; while for cohesive ligation, the PCR products were digested with Eco RI and Hind III restriction enzymes, resulting in a shorter insert size by about 124 bp each (Fig. 1A).

Processing by DROSHA, an RNAse III endonuclease, generates a 60 70-nt 60 70-ntp intermediate with a 3' overhang [ 6, 7].

In agreement with our prior data [ 38], a non-telomeric double-stranded DNA substrate with a 3' overhang is not degraded by WRN.

To examine in more details the kinetics of WRN-mediated processing of telomeric substrates with a 3' overhang, we titrated the amount of WRN added to the exonuclease reactions.

We have shown previously that a bifunctional oligonucleotide with a 5' overhang that contains binding sites for the hnRNP A1/A2 proteins strongly inhibits splicing when the oligonucleotide hybridizes immediately upstream of the 5' splice site of Bcl-xL (position -4 to -23).

These PCR products were tailed with A-overhangs as described above, cloned into pGEM-T, and sequenced.

This vector provides ready-to-use T-overhangs for ligation with A-overhangs of PCR-products.

Purified RT-PCR products were modified with A-overhangs by incubating with 10 mM dATP and 1u of Taq polymerase (Promega, Madison, USA) in 1× manufacturer's buffer for 10 mins at 72°C.

The ligation of synthetic dsDNA sequencing adapters (with 5' T overhang) to the fragment dsDNA (5' phosphorylated, with 3' A-overhang) again requires the use of DNA ligase, which is added in excess (concentration of 10 1) so as to ensure the attachment of as many adapters as possible per unit time.

The final 'T' at the fourth base anneals with the 'A' overhang from the end-repaired DNA sample for ligation of ChIP or other DNA fragments.