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A graded material was then generated, consisting of (i) a lower layer of the developed biomineralized collagen, corresponding to the subchondral bone, (ii) an upper layer of hyaluronic acid-charged collagen, mimicking the cartilaginous region, and (iii) an intermediate layer of the same nature as the biomineralized collagen, but with a lower extent of mineral, resembling the tidemark.
Similar to the HeLa cells, immunofluorescence staining for SBDS localization in PLB-985 cells revealed that SBDS protein is localized prominently to the nucleus with a lower extent of staining in the cytoplasm (Fig. 2D).
An overall decrease of αSMA and PDGFR- β expression was observed for MSCs, with a lower extent for the direct coculture condition and 3(d)).
In general, the lower lignin content in H58 concurs with a lower extent of substitution of plant cell wall polymers with phenolic acids: overall, the low-lignin-containing hybrid H58 contained 35 to 50% less phenolic acids than the RC, despite their similar xylan content (Table 1).
Cytotoxic T-cell cultures derived from mice primed with GC90/IRIV and boosted with each of the four PTH-rP peptides also showed a lytic capacity against both the targets, though with a lower extent for the CTL cultures derived from mice reboosted with PTR-1 peptide.
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Also in Sweden, women with atrial fibrillation have been treated with anticoagulants to a lower extent than men [ 31, 32].
The size reduction and collapse of GUVs incubated with RL16 and to a lower extent with RW16 suggested the capability of amphipathic peptides to permeabilize membranes.
Also, flotillin-2 was found to colocalize with Stx to a lower extent.
Only mdDC matured with flagellin, Pam3CSK4 and to a lower extent with FSL-1 additionally induced Th2 cells.
We found that discrimination between the mispaired C and 5mC templates is also observed with KOD exo-, albeitoto a lower extent than with KlenTaq (47 % extension of the A-primer when paired with 5mC template as compared to 34 % with C template; Figure 1 d).
This association is plausible: teachers with a fixed term contract tend to suffer the conflict with work-schedule to a lower extent, as they have time for training in between job spells or over the summer, or simply because they are more likely to indicate other non-time related barriers.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com