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Retained proteins were eluted with a linear gradient of acetonitrile 0-1000%).
rAaBGL1 was eluted with a linear gradient of NaCl (0 0.3 M).
The target protein was eluted with a linear gradient of 0-300 mM NaCl.
The run was continued with a linear gradient of 20 55% acetonitrile in 0.1% aqueous ammonium acetate for 20 min.
The adsorbed proteins were eluted with a linear gradient of 0-0.5 M sodium chloride in the same buffer.
The elution was carried out with a linear gradient of 25-45% acetonitrile with 0.1% trifluoroacetic acid.
The bound ABF-2 was eluted with a linear gradient of equilibration buffer with 0 600 mM NaCl.
The column temperature was programmed to increase from 40°C to 170°C with a linear gradient of 10°C/min.
His-tagged PnpE2 was eluted with a linear gradient of imidazole ranging from 10 mM - 200 mM in the buffer above.
Proteins bound to the column were eluted with a linear gradient of 0 0.2 mM NaCl in the same buffer.
Subsequently, the column was eluted with a linear gradient of 0 500 mM NaCl in 10 mM Tris HCl (pH 7.4).
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