Sentence examples for with a heparinized from inspiring English sources
Here we focus on whether filtration or surface heparin on AN69ST by a priming circuit with a heparinized saline contributes to HMGB1, with a heparin-binding protein, adsorption on AN69ST.
Cannulation of the abdominal aorta was done with a polyethylene catheter (0.5 mm, internal diameter, Folioplast, Sarcelles, France) and dripped with a heparinized serum (95% physiological serum, 2.5% xylocaine, and 2.5% heparin).
Mice were anesthetized under isofluorane 90 mins after toxin administration and whole blood was collected by cardiac puncture with a heparinized syringe.
Blood samples were obtained from direct puncture of the heart with a heparinized syringe and centrifuged at 2,400 g (4°C, 10 min).
The blood sampling procedure consisted in collecting a volume of 10 to 15 µl tail blood by capillarity with a heparinized 75-µl micro-haematocrit tube (Hirschmann Labogeräte).
Specifically, 10 hr after drug exposure for a given PCP or saline control treatment, mice were deeply anesthetized with sodium pentobarbital and perfused with a heparinized saline flush followed by 4% paraformaldehyde in a 0.1 M sodium phosphate buffer.
The samples were chosen randomly and through simple analysis according to a collecting protocol which consists of using 5 ml mixed venous blood, collected with a previously heparinized (0.1 ml heparin) syringe and with a total suction time of 20 s.
Blood samples (1 mL) were taken from the caudal vein with a 1.9 cm, 26-gauge needle and a syringe previously rinsed with a solution of heparinized saline to prevent coagulation (Houston 1990; Russell 1990).
Daily flushes with a solution of heparinized saline (10 U/ml) with streptokinase (0.67 mg/ml) and ticarcillin disodium (66.67 mg/ml) were performed.
Some rodents were also bled from the retroorbital sinus with heparinized capillary tubes.
Mice were anesthetized with a cocktail of ketamine/acepromazine, heparinized, and sequentially perfused via the left ventricle with 25 mL of PBS, 4% PFA, and a colored (red) contrasting agent MICROFIL® (Flow Tech Inc., Carver, MA, USA).
