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The mobile phase comprised of acetonitrile/water with a gradient system as follows: 0 min.
This last coil seizes 80% of its radial dimension with a gradient uniformity better than 5%.
The inverse problem relies on a classic local optimization approach implemented with a gradient method.
These observations demonstrate the utility of aligning a line-scan hyperspectral imager with a gradient material.
Localization is chosen to be controlled with a gradient plasticity approach.
The extraneous matrix interference was removed by flushing the extraction column with a gradient elution.
Fraction B was further purified by silica gel CC eluting with a gradient of CHCl3 MeOH (50:1–10:1), to yield five fractions (B1 B5).
The clarified lysate was loaded onto a 5 ml HisTrap column (GE Healthcare) and, after washing with the same buffer, eluted with a gradient to 300 mM imidazole.
The protein was then eluted with a gradient of imidazole in wash buffer (range 50 250 mM) with detection at 280 nm.
Fraction D was further purified by silica gel CC eluting with a gradient of CHCl3 MeOH (50:1–10:1), to yield five fractions (D1-D5).
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MRE images were collected with a gradient-echo technique gated to the transducer's motion.
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CEO of Professional Science Editing for Scientists @ prosciediting.com