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Lysates were probed with antibodies for Flag (to detect Flag-A51R/Flag-MVAA51R Flag-A51R/Flag-MVAA51R Flag-A51R/Flag-MVAA51R
Each seed dummy was marked with a flag to allow detecting the movement and burial of the dummies.
The membranes were probed with anti-Flag antibody to detect association of RNase II Flag with the RNaseE HA band.
The recombinant CHFR and its substrate proteins were immunoprecipitated from the corresponding cellular extracts with an anti-HA antibody and the ubiquitination activity levels were then monitored by western analysis with anti-FLAG antibodies to detect high molecular weight bands as described in a previous study [32].
This result was confirmed by immunoprecipitation using a FLAG-specific antibody to detect FLAG-tagged Cables1, followed by immunoblotting with a haemagglutinin (HA -specific antibody to detect HA -specificp63α.
Western blot analysis with anti-Flag antibody to detect ectopic TBX18 showed that the tumors developed nude mice expressed TBX18 (Figure 6D) suggesting that lack of inhibition of tumor growth in nude mice was not due to loss of TBX18 expression.
Panel 2: same co-immunoprecipitation experiment blotted with anti-FLAG antibodies to detect OSF-NEDD4L proteins.
Lysates from these cells were immunoblotted with anti-Flag antibodies to detect exogenous Nrf1.
Panel 3: The same co-immunoprecipitation experiment blotted with anti-FLAG antibodies to detect exogenously expressed OSF-NEDD4L.
Immunoprecipitates were then resolved by SDS-PAGE and western blotted with anti-FLAG antibodies to detect protein products of readthrough.
Proteins that were conjugated with His-Ub were pulled down by Ni2+-NTA beads followed by Western blotting with anti-flag antibodies to detect ubiquitin-conjugated BACE1.
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