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The DIG-labeled riboprobes were synthesized by T7 or SP6 polymerase with a DIG labeling mix (Roche) from a template containing the fragment from +4511 to +5159.
But the chancellor just had to accompany the support with a dig at Johnson's party.
And he finished with a dig at Lord Ashcroft, the Tory donor.
2012: Takes the Concacaf presidency with a dig at "improper" shamed predecessor Jack Warner.
Webb's Concacaf successor Alfredo Hawit – who took the job in May after Webb's arrest, with a dig at his ethics.
The comedian Jimmy Carr compounded Cameron's woes with a dig at the prime minister, via a tweet on Friday afternoon.
Similar(13)
The PCR products in the gel were then transferred onto nylon membranes and hybridized with a DIG-labeled probe of the cloned female-specific DNA fragment.
An antisense riboprobe for the AChRα subunit was synthesized with a DIG-labeling kit (Roche).
After washing in PBT, samples and sections were incubated in hybridization solution and then with a DIG-labelled probe (1 µg/ml) for 48 hours at 58°C.
Nylon membranes were crosslinked and then used for hybridization with a DIG-labeled probe.
Antisense and sense probes from linearized plasmid sequences were synthesized with a DIG-labeling kit (#11277073910, Roche Diagnostics).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com