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Moreover, this kind of model may leads to differential-algebraic equations with a differentiation index higher than one.
To differentiate the cells, the cells were cultured with a differentiation medium (DMEM containing 4.5 g/l glucose supplemented with 2% (v/v) FBS and 2 mM N,N-dibutyladenosine 3′,5′-phosphoric acid (dbcAMP; Nacalai Tesque Inc., Kyoto, Japan)).
Basis states are realized as polynomials in a space equipped with a differentiation inner product.
Names should be regularized using
Specifically, highly disturbed communities are expected to share the same dominant species across environmental gradients, while recovery from disturbance should be correlated with a differentiation among the dominant suite of species among sites.
In parenthesis, there are the p-values that correspond to those cases in which we remove the observations with a differentiation less than (Delta).
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Taken together, these data suggest that Tcf15 is associated with a differentiation-primed state that differs from the previously described primitive endoderm-primed subpopulation.
We found that genes including Klf4, Mili, Dazl and Stella were upregulated, whereas genes associated with a differentiation-primed state, such as Cldn6, Otx2, Lefty1/2 and Pitx2 [ 19], were downregulated (Fig 4A; supplementary Fig S3A,B online).
To do this, after cyclopamine treatment, cells were treated with a "dual" differentiation medium allowing the differentiation into adipocytes and osteoblasts.
Confluent preadipocytes were induced to differentiate with a standard differentiation medium (DM2-L1; Zen-Bio Inc., Research Triangle Park, NC, USA).
The Chip assay demonstrated that FCoR interacted with Cdkn1a in a differentiation time-dependent manner.
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