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In the second approach, miRNAs are first tailed with a common sequence and then reverse transcribed using a universal primer.
To ensure a uniform scale across simulations, we examine the average pairwise distance between SNPs with a common sequence pattern divided by the average pairwise distance over the entire tree.
HTS can also reduce the number of sequences needing to be tested in vitro, when coupled with bioinformatics, by identifying clusters of oligos with a common sequence or structure [ 28].
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The light exposure necessary to remove the NPPOC, Bz-NPPOC, and SPh-NPPOC protecting groups was determined by creating microarrays with oligonucleotides sharing a common sequence but synthesized using a gradient of light exposures.
In sum, all these evidences strongly suggest that cells with a mesodermal origin could require a common sequence of oncogenic events to become a tumor cells.
The results showed that 11.65 % total sRNAs (51.78 % unique sRNAs) with an average sequence mean frequency of 1.17 were specifically present in the 24 °C library, 6.9 % (32.92 % unique) with an average frequency of 1.09 were found only in the 37 °C library, and 81.45 % (15.3 % unique) with an average common sequence frequency of 27.78 were co-present in both libraries (Additional file 2).
It is noteworthy that recording of all variants was not conducted by comparison with a common reference sequence, such as the rCRS (revised Cambridge Reference Sequence), but rather by comparing the premutation node with the branch in which that mutation occurred.
This program mimics molecular evolution by producing a set of sequences along an evolutionary tree, starting with a common ancestral sequence at the root.
All three groups of chromosomes are compared with a common consensus sequence for B* 3906.
In many cases several of these could be associated with a common reference sequence using blast to query a reference database.
While Bowtie directly aligned about half of the collected reads to all the known transcript spliceform sequences, only 10% 'unique reads' identified spliceforms unambiguously (Table 1) because for targets with many common sequence regions, only a fraction of aligned reads will be discriminative.
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