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clade with a bootstrap value of 91%.
The BdPho was closely related to the phormicin of olive fruit fly (B. oleae) with a bootstrap value of 96%.
A phylogenetic tree of TCD10 and related proteins was then constructed by MEGA4 tool using the Neighbor-joining method with a bootstrap value of 1000 (Edgar 2004).
The monophyly of the Madagascan species clade is very well supported with a bootstrap value of 99 encompassing two distinct groups.
One of the unidentified isolates (Fanyuan1-11) was clearly clustered with 2 reference strains and the type strain of F. meridionale with a bootstrap value of 92%.
With a bootstrap value of 100%, this may suggest that Enterococcus spp. isolated from river water and riverbed sediment samples may be highly similar at the molecular level.
Figure 3d showed zlbR clustered closely to luxR family transcriptional regulator proteins from Pelagibaca abyssi and Thiobacimonas profunda with a bootstrap value of 53%.
Moreover, the phylogenetic tree constructed based on amino acid alignment (Fig. 3a, b) illustrated that zlaI clustered closely with an autoinducer synthase protein from Salipiger mucosus with a bootstrap value of 88% and zlbI was the least phylogenetically related to autoinducer synthase proteins from T. naphthalenivorans and Sagittula stellate with a bootstrap value of 52%.
However, this node is just weakly supported with a bootstrap value of <50%.
Group 2 consisted of 6 swine specimens which group together with a bootstrap value of 97%.
In contrast the STK35 like gene in Ciona species is grouped with STK35L1 homologs with a bootstrap value of 100%.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com